Key Documents

View All Documentation

C6745

Human Collagen Type IV

Name: Human Collagen Type IV
Brand: SIGMA

from human cell culture, liquid, 0.3 mg/mL, suitable for cell culture

Select a Size

Change View

About This Item

CAS Number:

9007-34-5

UNSPSC Code:

12352202

NACRES:

NA.75

EC Number:

232-697-4

MDL number:

MFCD00130825

Biological source:

human cell culture

Form:

solution

Technique(s):

cell culture | mammalian: suitable

Concentration:

0.3 mg/mL

Mol wt:

170—180 kDa

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

Collagen Type IV from human cell culture, Bornstein and Traub Type IV, 0.3 mg/mL, sterile-filtered, BioReagent, suitable for cell culture

biological source

human cell culture

Quality Level

200

sterility

sterile-filtered

product line

BioReagent

form

solution

mol wt

170—180 kDa

packaging

pkg of 1 mL

concentration

0.3 mg/mL

technique(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm²

impurities

HBSAG, none detected, HCV, none detected, HIV-1/HIV-2, none detected

NCBI accession no.

NM_001845.6

UniProt accession no.

P02462

binding specificity

Peptide Source: Laminin
, Peptide Source: Nidogen

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... COL4A1(1282)

Description

General description

Collagen IV, derived from human fibroblasts and epithelial cells in a co-culture system, creates an in vitro ECM (extracellular matrix) and serves as an excellent biological scaffold for three dimensional cell culture.

Collagen IV is a heterotrimeric molecules containing two α1-like and one α2-like chain. It is considered essential for completion of embryogenesis and is necessary for proper tissue organization and structural integrity. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types. Produced by human fibroblasts and epithelial cells, Collagen IV is then purified biochemically and provided as a sterile solution at a concentration of 0.3mg/ml in 25% acetic acid.

COL4A1 (collagen type IV α 1 chain) belongs to the collagen family. It has an N-terminal 7S domain, a C-terminal globular domain (NC1), and the central triple helical part with short interruptions of the Gly-X-Y repeats . It is an important constituent of type IV collagen in the basement membrane . The COL4A1 gene is located on human chromosome 13q34 .

Application

Collagen Type IV from human cell culture has been used in iPSC (induced pluripotent stem cells) culture and differentiation. It has also been used as membrane coatings.

Biochem/physiol Actions

COL4A1 (collagen type IV α 1 chain) related disorder is associated with pulmonary complication. This gene is a major constituent required in basal membrane stability. Mutations in COL4A1 result in familial porencephaly, infantile hemiplegia, cerebral small vessel disease (CSVD) and hemorrhagic stroke.

Features and Benefits

This Collagen Type IV product is generated by human fibroblasts and epithelial cells in a co-culture system, which produces an ECM in vitro.
The Collagen Type IV is then purified biochemically.
It is sterilized by dialysis in an acetic acid and 0.5% chloroform solution and is supplied sterile at a concentration of ~0.3 mg/ml in 25% acetic acid.

Still not finding the right product?

Explore all of our products under Collagen Type IV from human cell culture

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library