F2637
Ficoll® 400
BioXtra, lyophilized powder
Synonym(s):
Polysucrose 400, Ficoll 400
biological source
non-animal source
Quality Level
grade
Molecular Biology
product line
BioXtra
form
lyophilized powder
foreign activity
DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected
storage temp.
room temp
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General description
Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.
Application
Suitable for
- hybridization experiments to reduce non-specific binding of labeled probes to the membrane
- preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
- separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
- producing density gradients to separate subcellular components
- vitrification of cell and tissue samples.
- to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).
Used for cell separation and organelle isolation.
Features and Benefits
- free from DNases, RNases, phosphatases and proteases
- preserves functional and morphological integrity of the separated cells
Other Notes
A nonionic synthetic polymer of sucrose.
Legal Information
Ficoll is a registered trademark of Cytiva
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Optimization of isolated perfused/ventilated mouse lung to study hypoxic pulmonary vasoconstriction.
Hae Young Yoo et al.
Pulmonary circulation, 3(2), 396-405 (2013-09-10)
Hypoxic pulmonary vasoconstriction (HPV) is a compensatory physiological mechanism in the lung that optimizes the matching of ventilation to perfusion and thereby maximizes gas exchange. Historically, HPV has been primarily studied in isolated perfused/ventilated lungs; however, the results of these
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Preservation of cell and tissue samples from endangered species is a part of biodiversity conservation strategy. Therefore, setting up proper cell and tissue cryopreservation methods is very important as these tissue samples and cells could be used to reintroduce the
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Prion-like proteins form multivalent assemblies and phase separate into membraneless organelles. Heterogeneous ribonucleoprotein D-like (hnRNPDL) is a RNA-processing prion-like protein with three alternative splicing (AS) isoforms, which lack none, one, or both of its two disordered domains. It has been
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