G0762
β-Glucuronidase from Helix pomatia
Type H-3AF, aqueous solution, ≥60,000 units/mL
Synonym(s):
β-D-Glucuronide glucuronosohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥60,000 units/mL
Biological source:
Helix pomatia
biological source
Helix pomatia
Quality Level
type
Type H-3AF
form
aqueous solution
specific activity
≥60,000 units/mL
impurities
≤1,000 unit/mL Sulfatase
shipped in
wet ice
storage temp.
2-8°C
Application
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Biochem/physiol Actions
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Physical form
Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.
Analysis Note
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Other Notes
A further processing of Type H-3 to remove agglutinin.
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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W Lechner et al.
Acta endocrinologica, 109(2), 266-268 (1985-06-01)
Unconjugated and total oestriol (E3) have been determined in saliva (S) and plasma (P) by radioimmunoassay using a highly specific antiserum against E3. Total oestrogens (E = E1 + E2 + E3) in urine were analysed by the Kober-Ittrich method.
Tracey B Tucker Zhou et al.
The Journal of biological chemistry, 288(51), 36302-36311 (2013-11-13)
Klotho (KL) is an age-regulating protein named after the Greek goddess who spins the thread of life. Mice deficient in KL are normal throughout development, but rapidly degenerate and display a variety of aging-associated abnormalities that eventually lead to decreased
Regina Goetz et al.
Nature reviews. Molecular cell biology, 14(3), 166-180 (2013-02-14)
Fibroblast growth factors (FGFs) mediate a broad range of functions in both the developing and adult organism. The accumulated wealth of structural information on the FGF signalling pathway has begun to unveil the underlying molecular mechanisms that modulate this system