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Merck

G100120

Sephadex® G-100

Medium

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About This Item

CAS Number:
UNSPSC Code:
23151817
NACRES:
NA.56
MDL number:
Technical Service
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Quality Segment

form

powder

technique(s)

LPLC: suitable

matrix

Agarose

matrix active group

polymer

swelling

1 g swells to 15-20 mL gel

bead size

40-120 μm

separation technique

size exclusion (SEC)

General description

Sephadex® is composed of repeating glucose units attached by α-1,6 glucosidic bonds. This structure ensures more aptamer binding sites and greater binding capacity. It is suitable for large-scale purification and is considered stable and can be utilized more than once.
Fractionation Range (MW)
Globular Proteins: 4,000 - 150,000
Dextrans: 1,000 - 100,000

Application

Sephadex®G-100 is a gel filtration medium used in protein chromatography, affinity chromatography and gel filtration chromatography
It has been used:
  • in gel filtration column for α-amylase purification
  • in gel filtration column for the purification of polysaccharidic fraction extracted from the skin of the ray Raja radula
  • to remove excess protamine by size exclusion from cross-linked iron oxide (CLIO)-protamine-linked (B) peptides

Other Notes

G100120-100G′s updated product number is GE17-0060-01

Legal Information

Sephadex is a registered trademark of Cytiva


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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