G4134
Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)
Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)
Synonym(s):
G-6-P-DH, Zwischenferment
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-602-6
MDL number:
Specific activity:
200-400 units/mg protein (modified Warburg-Christian)
type
Type IX
Quality Level
form
lyophilized powder
specific activity
200-400 units/mg protein (modified Warburg-Christian)
mol wt
128 kDa
β-NADP and β-NADPH content
≤10 mmol/mol
application(s)
agriculture
shipped in
dry ice
storage temp.
−20°C
General description
Research area: Cell Signaling
Glucose-6-phosphate dehydrogenase (G6PD) is a key metabolic enzyme of the pentose phosphate pathway. In S. cerevisiae, it is encoded by the ZWF1 gene. G6PD exists as a tetramer in its active form.
Glucose-6-phosphate dehydrogenase (G6PD) is a key metabolic enzyme of the pentose phosphate pathway. In S. cerevisiae, it is encoded by the ZWF1 gene. G6PD exists as a tetramer in its active form.
Application
Glucose-6-phosphate dehydrogenase is used:
- To test ketose reductase activity in developing maize endosperm.
- For recycling microassay of β-NADP and β-NADPH.
- To measure the intracellular levels of NADPH and total NADP.
- To measure the nicotinamide adenine dinucleotide (NAD) kinase kinetic assay activity.
Biochem/physiol Actions
Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Glucose-6-phosphate dehydrogenase catalyzes the rate-limiting step in the pentose phosphate pathway. Its function involves the conversion of glucose-6-phosphate to 6-phosphogluconolacetone while generating NADPH, which is essential for the regeneration of glutathione The glutathione system utilizes nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) to effectively eliminate excess hydrogen peroxide. Glucose-6-phosphate dehydrogenase (G6PD) plays an important role in regulating cell growth and survival. Their levels are higher in cells undergoing normal and neoplastic growth. Increased glucose-6-phosphate dehydrogenase activity plays a pivotal role in preventing reactive oxygen species mediated cell death. Glucose-6-phosphate dehydrogenase is over expressed in several cancers whereas its activity is reduced in hyperglycemia. A deficiency in glucose-6-phosphate dehydrogenase causes hemolysis.
Physical form
Lyophilized powder essentially sulfate-free, containing approx. 20% sodium citrate
Other Notes
One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Instructions
The global prevalence of glucose-6-phosphate dehydrogenase deficiency: A systematic review and meta-analysis
Nkhoma ET and Poole C
Blood Cells, Molecules and Diseases, 42, 267?278-267?278 (2009)
The Pentose Phosphate Pathway in Yeasts-More Than a Poor Cousin of Glycolysis
Bertels LK, et al.
Biomolecules, 11(5), 725-725 (2021)
Impact of glucose-6-phosphate dehydrogenase deficiency on the pathophysiology of cardiovascular disease
Hecker PA, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 304(4), H491-H500 (2013)