G8404
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)
Synonym(s):
Entner-Doudoroff enzyme, G6PD, G6PDH, NADP glucose 6-phosphate dehydrogenase, G-6-P-DH
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥550 units/mg protein (biuret)
Biological source:
bacterial (Leuconostoc mesenteroides)
Recombinant:
expressed in E. coli
biological source
bacterial (Leuconostoc mesenteroides)
Quality Level
recombinant
expressed in E. coli
form
ammonium sulfate suspension
specific activity
≥550 units/mg protein (biuret)
mol wt
128 kDa
storage condition
(Tightly closed)
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
foreign activity
creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%
storage temp.
2-8°C
General description
Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).G6PD is a dimer and consists of a single active site at each subunit.
Research area: Cell Signaling
Research area: Cell Signaling
Application
Glucose-6-phosphate dehydrogenase has been used:
- to test ketose reductase activity in developing maize endosperm.
- to determine the levels of mannose in coronary heart disease patient-derived serum
- to study its activity on extracellular polymeric substance (EPS) extract to determine cell lysis through sonication
- to determine the glucose uptake in cultured human muscle satellite cells
Biochem/physiol Actions
G6PD provides all cells with reducing power as nicotinamide adenine dinucleotide phosphate (NADPH) which enables the cells to balance oxidative stress. Mutations in the G6PD gene are associated with X-linked G6PD deficiency, a hereditary genetic defect such as neonatal jaundice and acute hemolytic anemia.
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Physical form
Suspension in 3.2 M (NH4)2SO4 containing 50 mM Tris and 1 mM MgCl2, pH 7.5
Other Notes
One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.
The volume is lot specific and can be calculated using the data:
For example, lot 0000114274
Units per mg Protein = 846
Mg Protein per mL - 9
Units per mL = 7614
G8404-1000U = approx 132 ul
For example, lot 0000114274
Units per mg Protein = 846
Mg Protein per mL - 9
Units per mL = 7614
G8404-1000U = approx 132 ul
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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