L1378
L-Lactic Dehydrogenase from bovine muscle
Type X, ammonium sulfate suspension, ≥600 units/mg protein
Synonym(s):
(S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD, Lactate
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-617-8
MDL number:
Specific activity:
≥600 units/mg protein
Assay:
3.0—25.0 mg protein/mL (biuret)
Biological source:
bovine muscle
biological source
bovine muscle
Quality Level
type
Type X
assay
3.0—25.0 mg protein/mL (biuret)
form
ammonium sulfate suspension
specific activity
≥600 units/mg protein
manufacturer/tradename
Sigma-Aldrich
technique(s)
activity assay: suitable
color
white
pH
7.5
application(s)
life science and biopharma
foreign activity
pyruvate kinase ≤0.01%
storage temp.
2-8°C
Gene Information
cow ... LDHA(281274), LDHB(281275)
General description
Research Area: Cell Signaling
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.
Application
L-Lactic Dehydrogenase from bovine muscle has been used in lactate assay to assess lactate tissue accumulation.
Biochem/physiol Actions
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
Lactate dehydrogenase (LDH) plays a critical role as a checkpoint in gluconeogenesis and DNA metabolism. It plays a vital role in maintaining homeostasis during periods of low oxygen availability. As an oxidoreductase enzyme, lactate dehydrogenase catalyzes the reversible conversion of pyruvate to lactate using NADH. This enzyme is crucial for the anaerobic metabolism of glucose when oxygen is either absent or in short supply. LDH is the sole serum biomarker effective for evaluating metastatic melanomas.
Physical form
Crystalline suspension in 2.4 M (NH4)2SO4 solution, pH 6.0
Analysis Note
Protein determined by biuret.
Other Notes
One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
ppe
Eyeshields, Gloves, type N95 (US)
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