M9163
Medium 199
With Hanks′ salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X
Synonym(s):
M199 Medium, TCM 199
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Product Name
Medium 199, 10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: yes
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Preparation Note
Supplement with 0.1 g/L L-glutamine, 0.35 g/L sodium bicarbonate at 1×.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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