Key Documents

View All Documentation

P7367

PNGase F from Elizabethkingia meningoseptica

Name: PNGase F from <I>Elizabethkingia meningoseptica</I>
Brand: SIGMA

BioReagent, ≥95% (SDS-PAGE)

Synonym(s):

N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase

Select a Size

Change View

About This Item

CAS Number:

83534-39-8

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

3.5.1.52 (BRENDA, IUBMB)

MDL number:

MFCD18839214

Assay:

≥95% (SDS-PAGE)

Biological source:

bacterial (Elizabethkingia meningoseptica)

Concentration:

≥300 units/mL, ≥50 units/mL

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

bacterial (Elizabethkingia meningoseptica)

Quality Level

200

conjugate

(N-linked)

product line

BioReagent

assay

≥95% (SDS-PAGE)

form

powder

shelf life

≥1 weeks at 2‑8 °C (for a reconstituted solution >500 units/ml), ≥1 yr at 2‑8 °C, Solution is stable for at least 3 freeze-thaw cycles

mol wt

~36 kDa

concentration

≥300 units/mL, ≥50 units/mL

optimum pH

~8.6

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.

Application

PNGase F from Elizabethkingia meningoseptica has been used:

for de-N-glycosylation of Zika20virus E protein
to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells
to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)

Suitable for both proteomics and glycobiology use; compatible with MALDI-TOF MS analysis

Used to deglycosylate protein.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Other Notes

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Still not finding the right product?

Explore all of our products under PNGase F from Elizabethkingia meningoseptica

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Storage Class

11 - Combustible Solids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

0000492647

0000517842

0000492644

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library