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Merck

RAB0601

Human FGF1 / Fibroblast Growth Factor 1 ELISA Kit

for serum, plasma, cell culture supernatants and urine

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About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... FGF1(2246)
Input:
sample type urine
sample type cell culture supernatant(s)
sample type plasma
sample type serum
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type urine
sample type cell culture supernatant(s)
sample type plasma
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 12 pg/mL
standard curve range: 16.38-4000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FGF1(2246)

General description

FGF1 (fibroblast growth factor 1) gene encodes a member of the fibroblast growth factor (FGF) family. It encodes a pro-angiogenic protein that is ubiquitously expressed. In humans, FGF1 is alternatively spliced into four forms: FGF1A (in kidney), FGF1B (in brain), FGF1-C and -D (in vascular smooth muscle cells and fibroblasts). This gene is located on human chromosome 5q31.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

The FGF1 ELISA kit provides for the quantitative measurement of Fibroblast Growth Factor 1 in Cell Culture Supernatants, Plasma and Serum.
FGF1 (fibroblast growth factor 1) is mainly involved in cell growth, proliferation and neurogenesis. It also participates in wound healing, post-ischemic heart repair and making of collaterals after hindlimb ischemia. FGF1 functions as an angiogenic factor that participates in tissue repair, carcinogenesis and maintenance of vasculature stability.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Certificates of Analysis (COA)

Lot/Batch Number

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Related Content


Yash Chhabra et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(5), 6412-6430 (2019-02-20)
Growth hormone (GH) has an important function as an insulin antagonist with elevated insulin sensitivity evident in humans and mice lacking a functional GH receptor (GHR). We sought the molecular basis for this sensitivity by utilizing a panel of mice
Upregulation of fibroblast growth factor 1 in the synovial membranes of patients with late stage osteoarthritis.
Li R, et al.
Genetics and molecular research : GMR, 14(3), 11191-11199 (2015)
Regulation of FGF1 gene promoter through transcription factor RFX1.
Hsu YC, et al.
The Journal of Biological Chemistry (2010)