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Merck

V2260

Monoclonal Anti-V5-Peroxidase antibody produced in mouse

clone V5-10, purified immunoglobulin, lyophilized powder

Synonym(s):

Monoclonal Anti-V5

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:
Conjugate:
peroxidase conjugate
Clone:
V5-10, monoclonal
Application:
WB
Citations:
14
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biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

V5-10, monoclonal

form

lyophilized powder

packaging

vial of 0.5 mL

concentration

5-11 mg/mL

technique(s)

western blot: 1:4,000-1:8,000

storage temp.

2-8°C

General description

The V5 tag is a 14-amino acid long peptide sequence (GKPIPNPLLGLDST) that is derived from an epitope on the P and V proteins of simian virus 5 (SV5), which belongs to the paramyxovirus family. Monoclonal Anti-V5 Peroxidase conjugate reacts with V5-tagged recombinant fusion proteins expressed in transfected mmmalian cells or produced by in vitro translation.

Application

Suitable for immunoblotting.

Biochem/physiol Actions

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Physical form

Lyophilized from a 0.01 M phosphate buffered saline solution, pH 7.4, containing 1% BSA and 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Reconstitute with 0.5 mL of distilled water.


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signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Related Content


Panayotis C Theodoropoulos et al.
Nature chemical biology, 12(4), 218-225 (2016-02-02)
A hallmark of targeted cancer therapies is selective toxicity among cancer cell lines. We evaluated results from a viability screen of over 200,000 small molecules to identify two chemical series, oxalamides and benzothiazoles, that were selectively toxic at low nanomolar
Jordan N Ranum et al.
Nucleic acids research, 52(6), 3199-3212 (2024-02-26)
Productive infections by RNA viruses require faithful replication of the entire genome. Yet many RNA viruses also produce deletion-containing viral genomes (DelVGs), aberrant replication products with large internal deletions. DelVGs interfere with the replication of wild-type virus and their presence
Shuang Li et al.
iScience, 27(5), 109646-109646 (2024-04-19)
Most advanced colorectal cancer (CRC) patients cannot benefit from targeted therapy due to lack of actionable targets. By mining data from the DepMap, we identified FAM126B as a specific vulnerability in CRC cell lines exhibiting low FAM126A expression. Employing a