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Merck

E7510

Earle′s Balanced Salt Solution 10x

Without sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Earle’s Balanced Salts, EBSS

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
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Product Name

Earle′s Balanced Salt Solution 10x, Without sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: 10.0 g/L
NaHCO3: no
phenol red: 0.11 g/L

shipped in

ambient

Quality Level

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Application

Earle′s Balanced Salt Solution 10x has been used:
  • as an additive to the nutritive medium sheep nematode Trichostrongylus colubriformis eggs
  • in the swim-up method to prepare human sperm sample
  • in the steady-state medium to culture bovine primary mammary epithelial cells

Features and Benefits

  • Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance.
  • Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism.
  • When combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism.
  • Provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).

General description

Earle′s Balanced Salt Solution 10x is a saline solution with physiological pH. It is useful in inducing autophagy and promotes Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) maturation.

Preparation Note

Supplement with 2.2 g/L sodium bicarbonate at 1×.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Luchao Li et al.
Cell death & disease, 12(2), 220-220 (2021-02-28)
Receptor tyrosine kinase (RTK) inhibitors, such as sunitinib and sorafenib, remain the first-line drugs for the treatment of mRCC. Acquired drug resistance and metastasis are the main causes of treatment failure. However, in the case of metastasis Renal Cell Cancer
Yanli Ren et al.
Cancer letters, 390, 126-136 (2017-01-22)
Dyregulation of autophagy is implicated in human cancers and the mechanism details remains largely unclear. Herein we report the regulatory role of miR-638 in autophagy of esophageal squamous cell carcinoma (ESCC) and breast cancer cells. We found that miR-638 overexpression
A L Molan et al.
The Veterinary record, 150(3), 65-69 (2002-02-12)
The effects of condensed tannins extracted from seven forages on the viability of the eggs and first stage (L1) larvae of the sheep nematode Trichostrongylus colubriformis were evaluated in in vitro assays. The extracts of condensed tannins were obtained from
Yan Min et al.
Acta biochimica et biophysica Sinica, 45(3), 162-169 (2013-01-11)
Autophagy has been shown to enhance the efficacy of the Bacillus Calmette-Guérin (BCG) vaccine by increasing the peptide presentation of dendritic cells (DCs). Earle's balanced salts solution (EBSS) is a saline solution with physiological pH which is often used to
Siwen Yang et al.
Food & function, 11(1), 424-434 (2019-12-13)
Insulin resistance is the main cause of type 2 diabetes, fatty liver and obesity. Our previous study found that mushroom polysaccharides improved insulin resistance in vitro, but the underlying mechanisms were still unknown. Thus, we investigate the hypoglycemic effects of

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