O1391
Oil Red O solution
0.5% in isopropanol
Synonym(s):
Solvent Red 27, Sudan Red 5B
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About This Item
Empirical Formula (Hill Notation):
C26H24N4O
CAS Number:
Molecular Weight:
408.49
UNSPSC Code:
12171500
EC Number:
200-661-7
NACRES:
NA.47
form
liquid
Quality Level
concentration
0.5% in isopropanol
technique(s)
microbe id | staining: suitable
color
dark red
εmax
0.20-0.50 at 510-517 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
N(\N=C3\c4c(cccc4)C=CC\3=O)c1c(cc(c(c1)C)N=Nc2c(ccc(c2)C)C)C
InChI
1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-
InChI key
HJAYEODIXUYVIC-LTFAAIOASA-N
General description
Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.
Application
Oil red O solution has been used to stain neutral lipids and adipocytes and to visualize fat droplets in fibroblasts.
Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
target_organs
Central nervous system
Storage Class
3 - Flammable liquids
wgk
WGK 2
flash_point_f
53.6 °F - closed cup
flash_point_c
12.0 °C - closed cup
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LWW Doody's all reviewed collection, McClatchey: Clinical Laboratory Medicine
Clinical Laboratory Medicine (2002)