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Merck

B8756

Bile salts

suitable for microbiology

Synonym(s):

Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture

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About This Item

NACRES:
NA.85
UNSPSC Code:
41106212
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sterility

non-sterile

Quality Level

form

powder

solubility

distilled water: freely soluble

application(s)

microbiology

storage temp.

10-30°C

General description

Bile salt is an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Biles salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic and lithocholic acids.Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that enables isolation and identification of pathogens. For example, in MacConkey Agar/Broth.

Application

Bile salts may be used as one of the selective agents to prevent the growth of injured Escherichia coli cells, in an experiment done in order to identify the specific agent in the selective media which would inhibit the growth of the injured Escherichia coli ML30 cells. It may also be used for identification of isolates of Vibrio (Beneckea) vulnificus which is a halophilic bacterium, found responsible for causing several human infections.

Other Notes

Mixture of sodium cholate and sodium deoxycholate


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Daniela Andrea Ramirez et al.
Food chemistry, 215, 493-500 (2016-08-21)
Organosulphur compounds (OSCs) present in garlic (Allium sativum L.) are responsible of several biological properties. Functional foods researches indicate the importance of quantifying these compounds in food matrices and biological fluids. For this purpose, this paper introduces a novel methodology
Divya Eratte et al.
Food chemistry, 227, 129-136 (2017-03-10)
Solid co-microcapsules of omega-3 rich tuna oil and probiotic bacteria L. casei were produced using whey protein isolate-gum Arabic complex coacervate as wall material. The in-vitro digestibility of the co-microcapsules and microcapsules was studied in terms of survival of L.
Christina S Faherty et al.
Molecular microbiology, 85(1), 107-121 (2012-05-11)
Shigella flexneri is a Gram-negative pathogen that invades the colonic epithelium. While invasion has been thoroughly investigated, it is unknown how Shigella first attaches to the epithelium. Previous literature suggests that Shigella utilizes adhesins that are induced by environmental signals