A5295
Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Rabbit Anti-Bovine IgG (whole molecule)−HRP
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About This Item
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p)
Citations:
31
biological source
rabbit
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
bovine
technique(s)
direct ELISA: 1:20,000, dot blot: 1:80,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
IgG (immunoglobulin G) contributes to 10-20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.
Immunogen
purified bovine IgG
Application
Anti-Bovine IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- bovine viral diarrhea virus (BVDV) plaque
- focus-forming assays
Biochem/physiol Actions
Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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