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Merck

B4500

Nα-Benzoyl-L-arginine ethyl ester hydrochloride

trypsin substrate, chromogenic, ≥97% (HPLC), powder

Synonym(s):

BAEE

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About This Item

Empirical Formula (Hill Notation):
C15H22N4O3 · HCl
CAS Number:
2645-08-1
Molecular Weight:
342.82
NACRES:
NA.32
PubChem Substance ID:
24891777
UNSPSC Code:
12352204
EC Number:
220-157-0
MDL number:
MFCD00012579
Beilstein/REAXYS Number:
3781694

Key Documents

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Product Name

Nα-Benzoyl-L-arginine ethyl ester hydrochloride, trypsin substrate

InChI

1S/C15H22N4O3.ClH/c1-2-22-14(21)12(9-6-10-18-15(16)17)19-13(20)11-7-4-3-5-8-11;/h3-5,7-8,12H,2,6,9-10H2,1H3,(H,19,20)(H4,16,17,18);1H/t12-;/m0./s1

InChI key

HIXDELXKSSLIKB-YDALLXLXSA-N

SMILES string

Cl[H].CCOC(=O)[C@H](CCCNC(N)=N)NC(=O)c1ccccc1

assay

≥97% (HPLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless

storage temp.

2-8°C

Quality Level

200

General description

Nα-Benzoyl-L-arginine ethyl ester hydrochloride is a substrate for trypsin. It is a nonprotein substrate used in spectroscopic assays.
The prototype substrate for trypsin.

Application

  • N

  • α-Benzoyl-L-arginine ethyl ester hydrochloride has been used as a substrate:
  • in peptidyl arginine deiminase (PPAD) assay in P. gingivalis vesicles
  • for assaying proteolytic activity of enzymatic extracts of papaya plant parts
  • in trypsin activity assay of the tail tendon fascicles from rat

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000499113
0000499113
BCCN7609
BCCN5707
0000396931

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Kinetic and Structural Differentiation of Trypsin from Different Origins
Aithal M, et al.
BioPharm International, 30(1), 38-+-38-+ (2017)
Ying-Qing Yu et al.
Analytical chemistry, 75(21), 6023-6028 (2003-11-01)
Improved in-solution tryptic digestion of proteins in terms of speed and peptide coverage was achieved with the aid of a novel acid-labile anionic surfactant (ALS). Unlike SDS, ALS solubilizes proteins without inhibiting trypsin or other common endopeptidases activity. Trypsin activity
Mohammad K Hossain et al.
Colloids and surfaces. B, Biointerfaces, 181, 85-93 (2019-05-28)
Electric current responsive magnetic composite particles are prepared in three steps. In the first step, spherical and mesoporous submicrometer-sized magnetic iron oxide (Fe3O4) core particles are prepared by solvothermal method. Then magnetic Fe3O4 particles are functionalized with amine groups using
Chensong Pan et al.
Analytical and bioanalytical chemistry, 387(1), 193-204 (2006-11-07)
Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) is widely used in a variety of fields because it has the characteristics of speed, ease of use, high sensitivity, and wide detectable mass range for obtaining molecular weights and for structural characterization of
Maria T Dulay et al.
Analytical chemistry, 77(14), 4604-4610 (2005-07-15)
Trypsin is covalently linked to a photopolymerized sol-gel monolith modified by incorporating poly(ethylene glycol) (PSG-PEG) for on-column digestion of N(alpha)-benzoyl-l-arginine ethyl ester (BAEE) and two peptides, neurotensin and insulin chain B. The coupling of the enzyme to the monolith is
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