L1668
Lectin from Phaseolus vulgaris (red kidney bean)
Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture
Synonym(s):
Phaseolus vulgaris agglutinin, PHA
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)
Quality Level
product line
BioReagent
form
lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)
potency
≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)
technique(s)
cell culture | mammalian: suitable
color
white to off-white
solubility
PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)
storage temp.
2-8°C
Application
Lectin from Phaseolus vulgaris (red kidney bean) has been used:
- for the stimulation of cluster of differentiation (CD4+) T cells
- for the activation of primary peripheral blood lymphocytes
- for the stimulation of peripheral blood mononuclear cells
Biochem/physiol Actions
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
Packaging
Sold on the basis of lectin content
Analysis Note
Potency and solubility expressed in mg lectin/ml
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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