Anti-Mucin MUC5AC Antibody, clone CLH2

Mucin-5AC (UniProt: P98088; also known as MUC5-AC, Gastric mucin, Major airway glycoprotein 1, Mucin-5 subtype AC tracheobronchial, Tracheobronchial mucin 1. TBM) is encoded by the MUC5AC (also known as MUC5) gene (Gene ID: 4586) in human. MUC5-AC is a gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by mucociliary system. It is synthesized with a signal peptide (aa 1-27), which is later cleaved off to produce the mature form (aa 28-5654). MUC5-AC undergoes C-O- and N-glycosylated. O-glycosylation occurs on the second and last threonine of the threonine/serine-rich tandem repeats TTPSPVPTTSTTSA. C-mannosylation in the cysteine-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis. At its N- and C-termini are larger and more complex domains that are rich in cysteine and are involved in formation of the mucin polymers. These domains include sequences, including the D1, D2, D”, and D3 domains at the N-terminus and the D4, C, and CK domains at the C-terminus. MUC5-AC is overexpressed in a number of carcinomas. (Ref.: Pothuraju, R., et al. (2020). Mol. Cancer, 19: Article 37; Perez-Vilar, J., et al. (2004). Glycobiology. 14(4); 325-337).

Directed against a synthetic peptide of the MUC5AC tandem repeat.

Anti-Mucin MUC5AC Antibody, clone CLH2 detects level of Mucin MUC5AC & has been published & validated for use in ELISA, IP, WB, IC, IH(P).

Research Category
Apoptosis & Cancer

Research Sub Category
Tumor Markers

Western blot:Clone CLH2 shows a weak reactivity with a high molecular weight smear characteristic of mucin from a native mucin extract from normal gastric mucosa and from gastric diffuse carcinoma. Typical dilution is 1:100.

Immunocytochemistry

Immunohistochemistry. Effective on frozen and paraffin sections at 1:1000-1:2000 dilution.

ELISA

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Antibody MAB2011 is specific for the MUC5AC tandem repeat of human mucin. No cross reactivity with other peptides from MUC2, 3, 6, or 7.

Immunoreactivity of MAB2011 has been detected in the following normal human epithelial tissues: bronchus, endocervix, stomach, and gallbladder. The predominant staining pattern in normal tissues is perinuclear. Antibody reactivity has been observed in foveolar and mucopeptic neck cells of normal gastric mucosa. The expression of MUC5AC has been frequently observed in gastric carcinomas and may be used as a marker of gastric differentiation.

The CLH2 antibody shows a weak reactivity with a high molecular weight smear characteristic of mucin from native mucin extract from normal gastric mucosa and from gastric diffuse carcinoma .

The antibody was generated by immunizing mice with a synthetic tandem repeat peptide The antibody recognizes the sequence TTSTTSAP within the tandem repeat of MUC5AC (1). It further recognizes glycosylated as well as unglycosylated MUC5AC.

Format: Purified

Purified immunoglobulin. Liquid at 1 mg/mL in 0.02M phosphate buffer, 0.25 M sodium chloride, 0.1% sodium azide, pH 7.6.

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

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