324699
Elastase Substrate IV, Colorimetric
A specific substrate for pancreatic elastase.
Synonyme(s) :
Elastase Substrate IV, Colorimetric
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A propos de cet article
Formule empirique (notation de Hill) :
C25H34N6O9
Poids moléculaire :
562.57
NACRES:
NA.54
UNSPSC Code:
12352200
Assay:
≥95% (HPLC)
Form:
lyophilized solid
Storage condition:
OK to freeze, avoid repeated freeze/thaw cycles, desiccated
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assay
≥95% (HPLC)
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles, desiccated
color
white to off-white
solubility
ethanol: 1 mg/mL, water: soluble
shipped in
ambient
storage temp.
−20°C
General description
A specific substrate for pancreatic elastase (Km = 100 µM; Kcat/Km = 35,000 s-1 M-1 for rat pancreatic elastase; Km = 30 µM; Kcat/Km = 351,000 s-1 M-1 for porcine pancreatic elastase). Cleavage of substrate can be monitored at ~405 nm.
Suggested protocol: see Largman, C. (1983)
Suggested protocol: see Largman, C. (1983)
Biochem/physiol Actions
Cell permeable: no
Primary Target
A specific substrate for pancreatic elastase
A specific substrate for pancreatic elastase
Product does not compete with ATP.
Reversible: no
km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase; Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine
Physical form
45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.
Preparation Note
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
Other Notes
Largman, L. 1983. Biochemistry22, 3763.
Del Mar, E.G., et al. 1980. Biochemistry19, 468.
Del Mar, E.G., et al. 1980. Biochemistry19, 468.
Suc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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