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MAB2624

Anti-Endomucin Antibody, clone V.5C7

Name: Anti-Endomucin Antibody, clone V.5C7
Brand: MM

clone V.5C7, from rat

Synonyme(s) :

Endomucin-2, Gastric cancer antigen Ga34, Mucin-14, MUC-14, Endomucin-1/2

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A propos de cet article

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

V.5C7, monoclonal

Application:

FACS, IHC, IP, WB

Citations:

Service technique

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Propriétés

biological source

rat

Quality Segment

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

V.5C7, monoclonal

species reactivity

mouse

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001156994

UniProt accession no.

Q9R0H2

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Emcn(59308)

Description

General description

Endomucin is an endothelial sialomucin belonging to a class of glycoproteins characterized by their high levels of O-glycosolation and rich quantities of sialic acid. It inhibits focal adhesion assembly and hinders extracellular matrix and cell interaction. Observed expression is highest in kidney heart, brain, liver, lung, spleen, and thymus. Endomucin is only found in endothelial cells.

~ 75kDa observed

Immunogen

Mouse endothelial cells

Application

Anti-Endomucin Antibody, clone V.5C7 detects level of Endomucin & has been published & validated for use in WB, IP, IH, FC.

Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP of bEND cell lysate (Morgan, 1999).

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in immunhistochemical staining of frozen and paraffin-embedded tissue sections of mouse tongue, heart, uterus, brain, and small intestine (Morgan, 1999).

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC of transfected COS-7 cells (Morgan, 1999).

Research Sub Category
Organelle & Cell Markers

Adhesion (CAMs)

Physical form

Format: Purified

Analysis Note

Control
bEND3 cell lysate

Evaluated by Western Blot on mouse endothelioma cell line, bEND3, cell lysate.

Western Blot Analysis: 0.0025 µg/mL of this antibody detected Endomucin in 10 µg of bEND3 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c