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NA81

Anti-5-Methylcytosine Mouse mAb (162 33 D3)

liquid, clone 162 33 D3, Calbiochem®

Synonyme(s) :

Anti-5-mc, Anti-5-MeCyd

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A propos de cet article

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
162 33 D3, monoclonal
Species reactivity:
-
Application:
—
Citations:
56
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biological source

mouse

Quality Segment

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

162 33 D3, monoclonal

form

liquid

does not contain

preservative

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA (0.1 µg/mL)
Flow Cytometry (1 µg/mL)
Frozen Sections (5-10 µg/mL)
Immunoblotting (5 µg/mL)
Immunofluorescence (1 µg/mL)
Paraffin Sections (5-10 µg/mL)
Radioimmunoassay (0.1 µg/mL)
Southwestern Blot (5 µg/mL))

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Immunoaffinity purified mouse monoclonal antibody. Recognizes 5-methylcytosine.
Recognizes 5-methylcytosine in methylated DNA or RNA in NIH3T3 cells.

Specific
• Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications
• Flow Cytometry
• Frozen Sections
• Immunoblotting
• Immunofluorescence
• Paraffin Sections

Reliable
• Generates reproducible results.
• Clone 162 33 D3 has been used in over 50 publications.

This Anti-5-Methylcytosine Mouse mAb (162 33 D3) is validated for use in ELISA, FC, Frozen Sections, IF, Paraffin Sections, Radioimmunoassay, Southwestern Blot for the detection of 5-Methylcytosine.

Immunogen

5-methylcytosine conjugated to ovalbumin

Application



ELISA (0.1 g/ml)
Flow Cytometry (1 g/ml)
Frozen Sections (5-10 g/ml)
Immunoblotting (5 g/ml)
Immunofluorescence (1 g/ml)
Paraffin Sections (5-10 g/ml)
Radioimmunoassay (0.1 g/ml)
Southwestern Blot (5 g/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
NIH3T3 cells

Other Notes

Clone 162 33 D3 is useful for the quantitative and qualitative detection of methylated DNA or RNA in a variety of samples and applications. It has been used in over 40 publications. Antibody should be titrated for optimal results in an individual systems.
Ehrlich, M. 2002. Oncogene21, 5400. (Review)
Widschwendter, M. and Jones, P.A. 2002. Oncogene21, 5462. (Review)
Barton, S.C., et al. 2001. Hum. Mol. Genet.10, 2983.
Piyathilake, C.J., et al. 2001. Hum. Pathol.32, 856.
Taddei, A., et al. 2001. Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000. Biotech. Histochem.75, 251.
De Capoa, A., et al. 1999. FASEB J.13, 89.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Classe de stockage

11 - Combustible Solids

Que se passe-t-il ?

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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