PC253L
Anti-Calbindin D-28K (Ab-1) Rabbit pAb
lyophilized, Calbiochem®
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A propos de cet article
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
baboon, monkey, rat, human
Application:
—
Citations:
33
Service technique
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rabbit
Quality Segment
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
contains
≤0.1% sodium azide as preservative
species reactivity
baboon, monkey, rat, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Frozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence)
Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, )
isotype
IgG
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CALB1(793)
rhesus monkey ... Calb1(677721)
General description
Anti-Calbindin D-28K (Ab-1), rabbit polyclonal, reecognizes the Calbindin D-28K protein in rat hippocampus. It is validated for use in frozen sections and for T cell co-stimulation.
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes the calbindin D-28K protein.
Recognizes the Calbindin D-28K protein in rat hippocampus tissue.
Immunogen
Bovine
purified bovine cerebellum calbindin D-28K protein
Application
Frozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence)
Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)
Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)
Physical form
Undiluted serum.
Preparation Note
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Rat striatum, hippocampus, or cortex
Rat striatum, hippocampus, or cortex
Other Notes
Antibody specificity was examined in the rat striatum, cortex, and hippocampus. Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
Conde, F., et al. 1994. J. Comp. Neurol.341, 95.
Heizmann, C.W. 1993. Acta Neurobiol. Exp.53, 15.
Baimbridge, K.G., et al. 1992. Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci.16, 98.
Heizmann, C.W. 1993. Acta Neurobiol. Exp.53, 15.
Baimbridge, K.G., et al. 1992. Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci.16, 98.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Classe de stockage
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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