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PC253L

Anti-Calbindin D-28K (Ab-1) Rabbit pAb

lyophilized, Calbiochem®

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A propos de cet article

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
baboon, monkey, rat, human
Application:
—
Citations:
33
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biological source

rabbit

Quality Segment

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

contains

≤0.1% sodium azide as preservative

species reactivity

baboon, monkey, rat, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Frozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence)
Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, )

isotype

IgG

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CALB1(793)
rhesus monkey ... Calb1(677721)

General description

Anti-Calbindin D-28K (Ab-1), rabbit polyclonal, reecognizes the Calbindin D-28K protein in rat hippocampus. It is validated for use in frozen sections and for T cell co-stimulation.
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes the calbindin D-28K protein.
Recognizes the Calbindin D-28K protein in rat hippocampus tissue.

Immunogen

Bovine
purified bovine cerebellum calbindin D-28K protein

Application

Frozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence)

Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)

Physical form

Undiluted serum.

Preparation Note

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

Analysis Note

Positive Control
Rat striatum, hippocampus, or cortex

Other Notes

Antibody specificity was examined in the rat striatum, cortex, and hippocampus. Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
Conde, F., et al. 1994. J. Comp. Neurol.341, 95.
Heizmann, C.W. 1993. Acta Neurobiol. Exp.53, 15.
Baimbridge, K.G., et al. 1992. Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci.16, 98.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Classe de stockage

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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