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Merck

63069

Chlorure de magnésium solution

BioUltra, Molecular Biology, ~1 M in H2O

Synonyme(s) :

Magnesium Chloride hydrate, Magnesium chloride (MgCl2)hexahydrate, MgCl2

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A propos de cet article

Formule linéaire :
MgCl2
Numéro CAS:
Poids moléculaire :
95.21
NACRES:
NA.26
PubChem Substance ID:
UNSPSC Code:
12352302
MDL number:
Grade:
Molecular Biology
Form:
liquid
Solubility:
water: soluble
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grade

Molecular Biology

Quality Level

product line

BioUltra

form

liquid

concentration

~1 M in H2O

technique(s)

HPLC: suitable, flow cytometry: suitable

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.0002% total Nitrogen (N)

pH

5.0-7.5 (25 °C, 1 M in H2O)

solubility

water: soluble

density

1.08 g/mL at 20 °C

anion traces

phosphate (PO43-): ≤10 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤10 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤50 mg/kg, Li: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Na: ≤500 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.05, λ: 280 nm Amax: 0.03

suitability

suitable for molecular biology

application(s)

cell analysis
filtration
vaccine development

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

General description

Magnesium chloride is widely used in polymerase chain reactions (PCR) buffers to supply magnesium ions. The concentration of Mg2+ is the most critical parameter that impacts both the efficiency and specificity of PCR when total genomic DNA is used, synthesis of long products, or during multiplex reactions. Elevated concentrations of Mg2+ enhance the stability of primer-template duplexes and the efficiency of amplification.

Application

Magnesium chloride solution has been used in the reaction mix of reverse transcriptase enzyme, PFV RT. It has been used in the preparation of artificial cerebrospinal fluid (ACSF).
Magnesium chloride solution has been used as a component of nuclei isolation buffer for in situ fixations and isolation of nuclei from Kupffer cells (or other liver cells).

Other Notes

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Classe de stockage

12 - Non Combustible Liquids

ppe

Eyeshields, Gloves



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RNase H Polymerase-independent Cleavage Assay for Evaluation of RNase H Activity of Reverse Transcriptase Enzymes.
Corona A and Tramontano E
Bio-protocol null
Ty D Troutman et al.
STAR protocols, 2(1), 100363-100363 (2021-03-23)
Significant advancements in understanding disease mechanisms can occur through combined analysis of next-generation sequencing datasets generated using purified cell populations. Here, we detail our optimized protocol for purification of mouse hepatic macrophages (or other liver non-parenchymal populations) suitable for use
Benjamin Judkewitz et al.
Nature protocols, 4(6), 862-869 (2009-05-16)
In order to link our knowledge of single neurons with theories of network function, it has been a long-standing goal to manipulate the activity and gene expression of identified subsets of mammalian neurons within the intact brain in vivo. This