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Merck

35845

DCFHDA

BioReagent, suitable for fluorescence, ≥95% (HPLC)

Synonyme(s) :

2′,7′-Dichlorofluorescein diacetate, Diacetyldichlorofluorescein

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About This Item

Formule empirique (notation de Hill) :
C24H14Cl2O7
Numéro CAS:
2044-85-1
Poids moléculaire :
485.27
UNSPSC Code:
12352108
NACRES:
NA.32
PubChem Substance ID:
57649438
MDL number:
MFCD00037501
Beilstein/REAXYS Number:
68488

Nom du produit

DCFHDA, BioReagent, suitable for fluorescence, ≥95% (HPLC)

InChI key

VQVUBYASAICPFU-UHFFFAOYSA-N

InChI

1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3

SMILES string

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

Quality Level

100

description

suitable as a fluorogenic substrate for esterases

product line

BioReagent

assay

≥95% (HPLC)

form

powder or crystals

mol wt

485.27 g/mol

color

faint red
 white to faint brown

mp

232-234 °C (lit.)

solubility

DMSO: soluble

fluorescence

λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)

suitability

suitable for fluorescence

storage temp.

2-8°C

Catégories apparentées

Application

2′,7′-Dichlorofluorescein diacetate is predominantly used to detect reactive oxygen (ROS) intermediates and quantify the overall oxidative stress in cells.

Dcfh-DA is used in the quantitative assay to measure oxidative stress in nanoparticle-treated cells.

General description

2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
BCCN8736
BCCN2492
BCCL7891
BCCM2610
BCCL2220

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Consulter la Bibliothèque de documents

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is required to get 2´,7´-Dichlorofluorescein diacetate, Product 35845, to fluoresce?

    2',7'-dichlorofluorescein diacetate fluoresces only after cleavage of the acetyl groups.

  4. In what solvents is 2´,7´-´Dichlorofluorescein diacetate, Product 35845, soluble?

    It is soluble in DMF, ethanol (2 mg/mL), DMSO (10 mg/mL).

  5. What are the optical properties of the hydrolysis product of 2´,7´-Dichlorofluorescein diacetate, Product 35845?

    Excitation maximum is at 504 nm; emission maximum is at 529 nm (in 0.1 M Tris pH 8.0 following treatment with an esterase).

  6. What are the recommended storage conditions for 2´\,7´-Dichlorofluorescein diacetate, Product 35845?

    Product no. 35845 should be stored in a refrigerator or coldroom at 2-8°C.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Can Product 35845, 2´,7´-Dichlorofluorescein diacetate be used for ROS (Reactive Oxygen Species) detection?

    Yes, Product 35845, 2´,7´-Dichlorofluorescein diacetate can be used for ROS detection by FACS analysis. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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