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46043

Ethidium homodimer

Name: Ethidium homodimer
Brand: SIGMA

suitable for fluorescence, ~90% (HPCE)

Synonyme(s) :

EtDi, EthD-1

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A propos de cet article

Formule empirique (notation de Hill) :

C₄₆H₅₀Cl₄N₈

Numéro CAS:

61926-22-5

Poids moléculaire :

856.75

NACRES:

NA.32

PubChem Substance ID:

329757072

UNSPSC Code:

12161505

EC Number:

263-325-9

MDL number:

MFCD00210948

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Propriétés

Quality Segment

100

description

suitable for the fluorogenic detection of DNA

assay

~90% (HPCE)

form

solid

mol wt

856.75 g/mol

concentration

≤100% (5,5′-[Ethylenebis(iminotrimethylene)]bis[3,8-diamino-6-phenylphenanthridinium] dichloride dihydrochloride)

color

red to dark red

mp

≥250 °C (lit.)

solubility

DMF: soluble, DMSO: soluble, H₂O: soluble

fluorescence

λ_ex 493 nm in H₂O, λ_ex 528 nm; λ_em 617 nm in TE buffer; DNA

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28

InChI

1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H

InChI key

ZKAJUKABUUJRLB-UHFFFAOYSA-N

Description

Application

Ethidium homodimer (EtDi, EthD-1) is used as a staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. EthD-1 does not cross intact/viable cell membranes and can be used to test cell viability.

Staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. It will not cross intact cell membranes and can be used to test cell viability.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Reagent for the fluorimetric detection of nucleic acids

pictograms

GHS02,GHS08,GHS05

signalword

Danger

hcodes

H261,H314,H351

pcodes

P231 + P232 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Classe de stockage

4.3 - Hazardous materials which set free flammable gases upon contact with water

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c