F4135
Sérum de veau fœtal
USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma
Synonyme(s) :
FBS HI, FCS HI, Sérum HI, sérums HI, FBS, FCS, sérum, sérums
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About This Item
Nom du produit
Sérum de veau fœtal, USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma
biological source
bovine fetus
sterility
sterile-filtered
product line
FBS Classic
composition
Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL
origin
USA origin
technique(s)
cell culture | hybridoma: suitable
cell culture | insect: suitable
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Analysis Note
- A fait l'objet de tests pour rechercher la présence de bactéries, de virus et de mycoplasmes
- Filtré trois fois sur membrane de 0,1 micron en conditions aseptiques
- Taux d′endotoxines et d′hémoglobine testés
Application
Le sérum de veau fœtal a été utilisé :
- dans le milieu d'Eagle modifié par Dulbecco (DME ou DMEM), le milieu essentiel minimum d'Eagle (EMEM), le milieu RPMI 1640 (Roswell Park Memorial Institute), la modification α du milieu d'Eagle (αMEM) et le milieu L-15 de Leibovitz
- dans un milieu de blocage pour le traitement de coupes de tronc cérébral
- pour améliorer l'adhésion d'explants cutanés
General description
Preparation Note
Classe de stockage
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Does Sigma-Aldrich offer delipidated bovine serum?
At this time, Sigma-Aldrich does not offer delipidated bovine serum (either FBS or calf serum).A method for lipid depletion, though not necessarily that of Sigma:1. While mixing, gradually add 10 g fumed silica (Product S5130 or Aerosil) per liter of serum2. Mix at room temperature and then transfer to 2-8 °C for overnight mixing.3. After overnight mixing, remove flumed silica (Aerosil) by centrifugation at 3000-5000 rpm for 5 minutes.4. Retain the supernatant. The lipids should stick to the silica. There may be some protein loss
Why is serum Heat-Inactivated (HI)?
Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.
Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?
Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
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