L2005
Lactoperoxidase from bovine milk
lyophilized powder (essentially salt-free), ≥200 units/mg protein
Synonyme(s) :
Donor:hydrogen peroxide oxidoreductase, Peroxidase
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A propos de cet article
Numéro CAS:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-668-6
MDL number:
eCl@ss:
32160410
Specific activity:
≥200 units/mg protein
Biological source:
bovine milk
Service technique
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Laissez-nous vous aiderbiological source
bovine milk
Quality Segment
form
lyophilized powder (essentially salt-free)
specific activity
≥200 units/mg protein
absorbance ratio
A412/280 nm 0.7-0.9
UniProt accession no.
storage temp.
−20°C
SMILES string
[O+H2]O[O-]
InChI
1S/H2O3/c1-3-2/h1-2H
InChI key
JSPLKZUTYZBBKA-UHFFFAOYSA-N
Gene Information
cow ... LPO(280844)
General description
Lactoperoxidase is a major enzyme present in bovine milk and belongs to the peroxidase family. It is present in both plants and animals. The enzyme structure comprises a single polypeptide chain made up of 612 amino acid residues.
Application
Lactoperoxidase from bovine milk has been used as a standard for milk lactoperoxidase to study the role of H2O2 in the inhibition of Staphylococcus aureus growth by Lactococcus garvieae in the presence of lactoperoxidase in raw milk. It has also been used in lactoperoxidase-mediated iodination to prepare radiolabeled peptides.
Biochem/physiol Actions
Lactoperoxidase catalyzes the oxidation of iodide to iodine by hydrogen peroxide. This activity provides a gentle, specific alternative to chloramine T for the radioiodination of proteins and DNA.
Lactoperoxidase contributes to the antimicrobial system of milk by inactivating a wide range of micro-organisms. This lactoperoxidase-mediated antimicrobial system is also identified in human secretions such as tear-fluid, saliva, and milk. Lactoperoxidase catalyzes the oxidation of molecules by releasing H2O2. The product exhibits antimicrobial activity.
Analysis Note
Protein determined by Lowry method.
Other Notes
One unit will oxidize 1.0 μmole of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 5.5 at 25 °C.
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Danger
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Hazard Classifications
Resp. Sens. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
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