L3126
Lipase from porcine pancreas
Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
Synonyme(s) :
Pancreatic lipase, PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-619-9
MDL number:
Specific activity:
≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
Biological source:
Porcine pancreas
Service technique
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Porcine pancreas
Quality Segment
type
Type II
form
lyophilized (cake)
specific activity
≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
composition
protein, ≥20%
storage temp.
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
Application
Lipase from porcine pancreas has been used:
- for the analysis of fatty acid at the 2-position of triglycerides.
- to determine the indigestible fraction of plant source that is resistant to the action of digestive enzymes.
- as a component of dissolution media to study the dissolution profiles of theophylline.
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Biochem/physiol Actions
Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Analysis Note
Crude (Steapsin). Contains amylase and protease activity.
Protein determined by biuret.
Other Notes
One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)
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Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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