Puromycine dihydrochloride from Streptomyces alboniger

Chemical structure: peptidyl nucleoside

Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C.

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Recommended for use as a selection agent at a range of 1-10 μg/mL.

Mode d′action : La puromycine inhibe la synthèse des protéines en provoquant la terminaison prématurée de la chaîne, en servant d′analogue à l′extrémité 3′-terminale de l′aminoacyl-tRNA.

Mode de résistance : La puromycine acétyltransférase est un gène de résistance efficace.

Spectre antimicrobien : Ce produit est actif contre les micro-organismes à Gram positif, moins actif contre les bacilles acidorésistants et faiblement actif contre les micro-organismes à Gram négatif. La puromycine peut empêcher la croissance des bactéries, protozoaires, algues et cellules de mammifères et agit rapidement, tuant 99 % des cellules dans un délai de 2 jours.

Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL.

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This product is soluble in water at 50 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C. This product is also soluble in methanol at 10 mg/mL.

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place. Keep in a dry place

As supplied, this product remains active for four years when stored at -20°C.