Chromolith® RP-18 Endcapped HPLC Columns |
The chemical basis of Chromolith® RP-18 endcapped HPLC columns – from starting materials to surface modification procedures – is the same as with high-end, conventional packed columns. Therefore, their selectivity is comparable to high-quality C18 endcapped packed reversed-phase columns. This allows the use of standard methods when developing new protocols.
The difference is that Chromolith® columns for reversed-phase liquid chromatography are based on high-purity silica, hence they minimize the negative effect of trace metals. Furthermore, the columns are chemically modified with n-alkyl chains which possess a high ligand density, and fully endcapped in order to reduce the effect of unmodified silanol groups.
Features and Benefits
- High throughput at high flow rates with the best overall column quality
- Possibility of flow gradients
- Added column performance by column coupling
- A rigid monolithic structure for longer column lifetime
- Lower matrix sensitivity
Technical Data
| Silica Type | High-purity |
|---|---|
| Particle Size | Monolithic |
| Macropore Size | 1.5 μm (2 mm i.d. columns) 2 μm (25, 10, 4.6 and 3 mm i.d. columns) |
| Mesopore Size | 13 nm (130 Å) |
| Pore Volume | 1 mL/g |
| Total Porosity | > 80% |
| Surface Area | 300 m2/g |
| Surface Modification | RP-18 endcapped |
| Carbon Content | 18% |
Column Lengths
Leading in high-speed separations, Chromolith® RP-18 endcapped columns are available in various lengths:
Product |
Internal Diameter |
Ordering Number |
|---|---|---|
| Chromolith® 25 mm For ultra-fast separation of simple mixtures Chromolith® Flash RP-18 endcapped is among the fastest HPLC column on the market. Due to the very short length of the column, its number of theoretical plates is sufficient for easy separations. |
25 x 2 mm 25 x 3 mm 25 x 4,6 mm |
152014 152003 151463 |
| Chromolith® 50 mm For fast separation of simple mixtures These short, fast columns are not only perfect for rapid screening of samples during in-process controls, but also for laboratories specialized in research or organic synthesis (e.g. combinatorial chemistry). |
50 x 2 mm |
152007 152002 151450 |
| Chromolith® 100 mm For rapid separation of more complex mixtures This column is perfect for use as a routine analytical tool in quality control laboratories, or in research laboratories where more complex mixtures are being analyzed. |
100 x 2 100 x 3 mm 100 x 4,6 mm |
152006 152001 102129 |
| Validation Kit For accurate method validation, it is essential to assess all possible sources of variations. To assist this process, the Chromolith® Performance RP-18 endcapped validation kit includes three columns from three different production batches, allowing you to compare batch-to-batch reproducibility and quality. This makes the validation kit the ideal tool for quality control and validation laboratories. |
2 mm 3 mm 4,6 mm |
152062 152063 151466 |
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