Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Merck’s PluriSTEM™ Human ES/iPS Medium is a specially formulated defined media that maintains human pluripotent stem cells in feeder-free and serum-free conditions with less frequent feeding and cell culture time. The proprietary formulation utilizes Activin-A, TGFβ1, and b-FGF to promote stem cell self renewal and potent small molecule combinations to inhibit unwanted spontaneous differentiation along with HSA to aid in overall colony morphology.
Suggested feeding regiment with Pluristem™ Human ES/iPS Medium (Product Number SCM130). H9 human ES cells cultured following the above regiment display undifferentiated morphology after 30 passages and express the pluripotent markers SSEA-4 and Oct-4.
Human ES Cells (H9) grown on PluriSTEM™-XF Recombinant Vitronectin (CC130) in PluriSTEM™ Human ES/iPS Medium (SCM132).
PluriSTEM™-XF Recombinant Vitronectin
Move Over Matrigel.
PluriSTEM™ Recombinant Vitronectin is a truncated version of human vitronectin protein and is an effective xeno-free alternative to Matrigel for human ES and iPS cell culture using PluriSTEM™ Human ES/iPS Medium, E8, MTESR or NutriStem human ES cell growth media in feeder-free culture conditions.
Dispase-II has proven to be a rapid and effective, yet gentle, agent for separating many tissues and cells grown in vitro. Reliably passage your human ES/iPS cells with our ready to use Dispase-II Enzymatic solution proven to work alongside PluriSTEM™ Human ES/iPS Medium.
Why waste time and energy with low yield pluripotent cell cryopreservation? Efficiently cryopreserve your human ES/iPS cells with our specially formulated PluriSTEM™ Freezing Medium.