Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Molecular weight standards (lanes 1, 3, 5, 7) and calf liver lysate (lanes 2, 4, 6, 8) were transferred to Immobilon®-P or Immobilon®-PSQ membranes. A sheet of Immobilon®-PSQ transfer membrane was placed behind the primary membranes to capture proteins that passed through (lanes 5 and 6 behind Immobilon®-P; lanes 7 and 8 behind Immobilon®-PSQmembrane).