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Key Publications and Guidances on EVs

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Title: Extracellular Vesicle Enrichment and Characterization from Cell Culture Supernatants and Biological Fluids

Presenter: Dr. Aled Clayton, Exosome Biology Group, Cardiff University

Presenter: Dr. Amedeo Cappione III, Senior Scientist, Merck

Date: Thursday, May 21, 2015

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Read This Before You Start Your Extracellular Vesicle Research!

EV preparations can vary in molecular composition and function, depending on:
  • Cell culture viability
  • Apoptotic index
  • Cell proliferation rate
  • Status of the cell culture medium
  • Handling conditions
  • Other experimental parameters
To support EV researchers trying to navigate this rapidly changing field, we've made available to you a suite of resources for your easy reference.
  1. Wall Poster: Preparing functional extracellular vesicles (EVs): Join the rEVolution.

    Hang this unique poster in your lab to be reminded of EV biogenesis pathways, cell type-specific EV processes, and characteristic protein and miRNA markers.


  2. Recorded Presentation from the American Association for Cancer Research (AACR) 2015 Annual Meeting: Strategies for enrichment, quantitation, and characterization of microvesicles for cancer research

    In this 45-minute presentation, learn best practices for standardizing and reducing the EV preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles. We will show how imaging flow cytometry improves the analysis and characterization of these microvesicles in a quantitative and reproducible manner above and beyond the current methods of analysis.

  3. Review article in BioProcessing Journal (in press): Preparing biotherapeutic extracellular vesicles: ultrafilters, MSCs and the regulatory horizon.

  4. Detection of internalized exosomes in single cancer cells using Amnis® imaging flow cytometry: View Poster Presented at CYTO 2013

    Exosomes were co-cultured with tumor cells and analyzed for internalization of PKH26+ particles in the tumor cell lines using the Amnis ImageStream®X imaging flow cytometer.

ISEV Guidances: Quick Links

The International Society for Extracellular Vesicles (ISEV) has published guidances for preparation and analysis, recognizing that the exact methods could depend on the scientific question being asked.

Read the most recent guidances:

Witwer KW et al. Standardization of sample collection, isolation and analysis methods in extracellular vesicle research. 2013 May 27;2.

Lötvall J et al. Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles. 2014 Dec 22;3:26913.