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  • P2X3 receptors mediate visceral hypersensitivity during acute chemically-induced colitis and in the post-inflammatory phase via different mechanisms of sensitization. 25885345

    Experiments using P2X3 knock-out mice or more general P2X receptor antagonists suggest that P2X3 receptors contribute to visceral hypersensitivity. We aimed to investigate the effect of the selective P2X3 antagonist A-317491 on visceral sensitivity under physiological conditions, during acute colitis and in the post-inflammatory phase of colitis.Trinitrobenzene sulphonic-acid colitis was monitored by colonoscopy: on day 3 to confirm the presence of colitis and then every 4 days, starting from day 10, to monitor convalescence and determine the exact timepoint of endoscopic healing in each rat. Visceral sensitivity was assessed by quantifying visceromotor responses to colorectal distension in controls, rats with acute colitis and post-colitis rats. A-317491 was administered 30 min prior to visceral sensitivity testing. Expression of P2X3 receptors (RT-PCR and immunohistochemistry) and the intracellular signalling molecules cdk5, csk and CASK (RT-PCR) were quantified in colonic tissue and dorsal root ganglia. ATP release in response to colorectal distension was measured by luminiscence.Rats with acute TNBS-colitis displayed significant visceral hypersensitivity that was dose-dependently, but not fully, reversed by A-317491. Hypersenstivity was accompanied by an increased colonic release of ATP. Post-colitis rats also displayed visceral hypersensitivity that was dose-dependently reduced and fully normalized by A-317491 without increased release of ATP. A-317491 did not modify visceral sensitivity in controls. P2X3 mRNA and protein expression in the colon and dorsal root ganglia were similar in control, acute colitis and post-colitis groups, while colonic mRNA expression of cdk5, csk and CASK was increased in the post-colitis group only.These findings indicate that P2X3 receptors are not involved in sensory signaling under physiological conditions whereas they modulate visceral hypersensitivity during acute TNBS-colitis and even more so in the post-inflammatory phase, albeit via different mechanisms of sensitization, validating P2X3 receptors as potential new targets in the treatment of abdominal pain syndromes.
    Document Type:
    Reference
    Product Catalog Number:
    AB5895

  • P2X3 CONTROL PEPTIDE -2753260

    Document Type:
    Certificate of Analysis
    Lot Number:
    2753260
    Product Catalog Number:
    AG356
    Product Catalog Name:
    P2X3, control peptide for AB5895 and AB5896

  • P2X3 CONTROL PEPTIDE - 3823301

    Document Type:
    Certificate of Analysis
    Lot Number:
    3823301
    Product Catalog Number:
    AG356
    Product Catalog Name:
    P2X3, control peptide for AB5895 and AB5896

  • P2X3 CONTROL PEPTIDE

    Document Type:
    Certificate of Analysis
    Lot Number:
    3098845
    Product Catalog Number:
    AG356
    Product Catalog Name:
    P2X3, control peptide for AB5895 and AB5896

  • P2X3 CONTROL PEPTIDE - 3940597

    Document Type:
    Certificate of Analysis
    Lot Number:
    3940597
    Product Catalog Number:
    AG356
    Product Catalog Name:
    P2X3, control peptide for AB5895 and AB5896