1.46476
ReadyTube® Half Fraser Broth
ReadyTube®, bottle capacity 250 mL, bottle filling volume 225 mL, closure type, blue screw cap with 3 loci, box of 6 bottles, selective for Listeria monocytogenes
Synonym(s):
Fraser Broth
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.74
UNSPSC Code:
41106212
Bottle capacity:
250 mL
Bottle filling volume:
225 mL
Feature:
closure type blue screw cap with 3 loci, ready-to-use
Agency:
ISO 11190
Ph:
7.2 ( in H2O)
agency
ISO 11190
Quality Level
form
liquid (ready-to-use)
feature
closure type blue screw cap with 3 loci, ready-to-use
packaging
box of 6 bottles
manufacturer/tradename
ReadyTube®
bottle capacity
250 mL
bottle filling volume
225 mL
pH
7.2 ( in H2O)
application(s)
environmental
food and beverages
microbiology
storage temp.
2-8°C
suitability
selective for Listeria monocytogenes
General description
ReadyTube® is ready to use media to provide the highest level of quality and testing confidence for improving accuracy and significantly reducing the risk of false results. ReadyTube® Half Fraser broth is a selective enrichment medium designed to help recovery of Listeria sp.
Application
ReadyTube® Fraser broth is used for the primary and secondary selective enrichment of Listeria sp. from food and animal feed as well as from environmental samples and other materials.
Analysis Note
This medium contains peptone, casein, yeast extract, and meat extract, which provide essential nutrients like carbon and nitrogenous compounds, including vitamins, amino acids, and trace ingredients. Phosphates buffer the medium while sodium chloride maintains osmotic equilibrium. Nalidixic acid and acriflavine inhibit the growth of Gram-negative and Gram-positive organisms respectively, except Listeria sp. Listeria sp. hydrolyze esculin to glucose and esculetin. The latter combines with ferric ions of ferric ammonium citrate, resulting in the formation of 6-7-Dihydroxycoumarin, a black-brown complex. Ferric ammonium citrate also enhances the growth of L. monocytogenes. The high salt tolerance (of sodium chloride) of Listeria is used as means to inhibit the growth of Enterococci. Lithium chloride is also used to inhibit Enterococci, which also possess the ability to hydrolyze esculin.
Legal Information
READYTUBE is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.