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Merck

361527

PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3)

liquid, clone 2D3, Calbiochem®

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Nombre del producto

PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3), liquid, clone 2D3, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

2D3, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative (antibody only)

species reactivity

mouse, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(ELISA (0.05 µg/mL)
Immunoblotting (0.5 µg/mL))

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer9)

Quality Level

100

Gene Information

human ... GSK3B(2932)
mouse ... Gsk3B(56637)

General description

Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
This PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3) is validated for use in ELISA, Immunoblotting for the detection of GSK3β (pSer⁹).

Immunogen

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β

Analysis Note

Positive Control
Pervanadate-treated HepG2 (included)

Application

ELISA (0.05 µg/ml)

Immunoblotting (0.5 µg/ml)

Disclaimer

Toxicity: Standard Handling (A)

Other Notes

For immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.

Preparation Note

Reconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Adam R Cole et al.
Methods in molecular biology (Clifton, N.J.), 468, 45-65 (2008-12-23)
Glycogen synthase kinase (GSK)-3 is a key signalling intermediate in the action ofWnts. This protein kinase is ubiquitously expressed and has high inherent activity but is inhibited by activation of Wnt signalling or activation of growth factor receptor tyrosine kinases

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