389561
Hyaluronidase, Streptomyces hyaluronlyticus nov. sp.
Sinónimos:
Hyaluronidase, Streptomyces hyaluronlyticus nov. sp.
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Número CAS:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
Specific activity:
≥2000 units (A280)
Servicio técnico
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Permítanos ayudarleconjugate
conjugate (Glucosaminoglycan)
Quality Level
description
Merck USA index - 14, 4758
form
lyophilized powder
specific activity
≥2000 units (A280)
packaging
ampule of ≥100 U (1 U = 1 TRU (Turbidity Reducing Unit))
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white
solubility
0.5 M acetate, pH 6.0: 30 U/mL, water: 30 U/mL
foreign activity
protease ≤0.05 U/ampule (pH 6.0)
shipped in
ambient
storage temp.
2-8°C
General description
Native hyaluronidase from Streptomyces hyaluronlyticus nov. species. Catalyzes the degradation of hyaluronic acid. Produces δ4,5-unsaturated tetra- and hexasaccharides.
Please Note: The appearance of this product can vary from gray cottony fibers to balled up droplets with no change in quality.
Please Note: The appearance of this product can vary from gray cottony fibers to balled up droplets with no change in quality.
Preparation Note
Following reconstitution, store in the refrigerator (4°C). Stock solutions prepared in water are stable for up to 2 weeks at 4°C and stock solutions prepared in 500 mM acetate buffer, pH 6.0, are stable for up to 1 month at 4°C.
Other Notes
Baker, J.R., et al. 2002. Biochem. J.365, 317.
Mogelerskii, M.S. 1964. Fed. Proc. 23, T559.
Mogelerskii, M.S. 1964. Fed. Proc. 23, T559.
One TRU (Turbidity Reducing Unit = 1 U) is defined as the amount of enzyme that will hydrolyze 50% of hyaluronic acid, determined by a 50% reduction in turbidity, in 30 min at 60°C pH 6.0.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Jennifer E Rowley et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 68(1), 75-91 (2019-11-13)
Hyaluronan (HA) is a ubiquitous component of the extracellular matrix. The spatial-temporal localization of HA can be visualized in situ using biotinylated HA binding proteins (HABPs). This assay is sensitive to fixation conditions, and there are currently no best practices