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Anti-KDEL Mouse mAb (10C3)

liquid, clone 10C3, Calbiochem®

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NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
10C3, monoclonal
Species reactivity:
mouse, sheep, canine, hamster, Drosophila, rat, guinea pig, human, monkey, yeast, rabbit, porcine
Application:
—
Citations:
7
Servicio técnico
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biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

10C3, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, sheep, canine, hamster, Drosophila, rat, guinea pig, human, monkey, yeast, rabbit, porcine

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (4 µg/mL, colorimetric)
Immunocytochemistry (5 µg/mL)
Immunoprecipitation (5 µg/mL))

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Kdelr1(68137)

General description

Protein G purified mouse monoclonal antibody. Recognizes the KDEL Grp78 (BiP) and Grp94 proteins.
Recognizes the Grp78 (BiP) and Grp94 proteins.
This Anti-KDEL Mouse mAb (10C3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of KDEL.

Immunogen

Rat
a synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH

Application

Immunoblotting (4 µg/ml, colorimetric)

Immunocytochemistry (5 µg/ml)

Immunoprecipitation (5 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 50% glycerol.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
RK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells

Other Notes

Also recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.
Kuznetsov, G., et al. 1994. J. Biol. Chem.269, 22990.
Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
Munro, S., and Pelham, H.R.B. 1987. Cell48, 899.
Munro, S., and Pelham, H.R.B. 1986. Cell46, 291.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Highly Toxic (H)

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Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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