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Merck

MAB1340

Anti-Collagen Type I (COL1A1) Antibody

CHEMICON®, mouse monoclonal, C11

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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
C11, monoclonal
Species reactivity:
human
Application:
ELISA, IHC
Citations:
11
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Nombre del producto

Anti-Collagen Type I Antibody, clone C11, ascites fluid, clone C11, Chemicon®

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

C11, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunohistochemistry: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... COL1A1(1277)

Application

Detect Collagen Type I using this Anti-Collagen Type I Antibody, clone C11 validated for use in ELISA, IH.
Immunohistochemical staining of 10 μm thick cryostat sections fixed with acetone or ethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Suggested working concentration 10-20 μg/mL. Formaldehyde fixation is not recommended. Does not work with Western blots. Not suitable for use on paraffin embedded sections.

ELISA: Apparent binding constant from titration curves is approximately 0.1nM. Antibody suitable for use in sandwich ELISA for specific determination of collagen I, employed as either a capture antibody or as a second, detecting antibody.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes both native and heat denatured human collagen type I. No cross reactivity with albumin fractions of human serum or albumins of other animal species. Less than 5% binding to collagens II and II in ELISA, RIA or immunoblotting.

Physical form

Immunoglobulin Purified from ascites by ammonium sulfate precipitation and chromatography on DEAE-cellulose. . Lyophilized, reconstitute with 1 mL of distilled water.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


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Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects



Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence.
Zoumi, A; Yeh, A; Tromberg, BJ
Proceedings of the National Academy of Sciences of the USA null
Patricia Khashayar et al.
3 Biotech, 7(5), 312-312 (2017-09-29)
This article explains a step-wise protocol to develop an electrochemical sensor to quantify serum levels of C-telopeptide (CTX) crosslinks also known as crosslaps in a matter of minutes and with high level of accuracy. The technique needs only one-step (incubation)
Jose M Gonzalez et al.
Scientific reports, 6, 21315-21315 (2016-02-18)
The contractile trabecular meshwork (TM) modulates aqueous humor outflow resistance and intraocular pressure. The primary goal was to visualize and quantify human TM contractile state by analyzing actin polymerization (F-actin) by 2-photon excitation fluorescence imaging (TPEF) in situ. A secondary



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SKUGTIN
MAB134004053252358852