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Merck

77607

Fenol

BioUltra, Molecular Biology, TE-saturated, ~73% (T)

Sinónimos:

Hidroxibenceno

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Fórmula lineal:
C6H5OH
Número CAS:
Peso molecular:
94.11
UNSPSC Code:
12352104
NACRES:
NA.25
PubChem Substance ID:
EC Number:
203-632-7
Beilstein/REAXYS Number:
969616
MDL number:
Assay:
~73% (T)
Grade:
Molecular Biology
Bp:
182 °C (lit.)
Vapor pressure:
0.09 psi ( 55 °C), 0.36 mmHg ( 20 °C)
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grade

Molecular Biology

Quality Level

vapor density

3.24 (vs air)

vapor pressure

0.09 psi ( 55 °C), 0.36 mmHg ( 20 °C)

product line

BioUltra

assay

~73% (T)

form

liquid

autoignition temp.

1319 °F

quality

TE-saturated

contains

~0.1% 8-hydroxyquinoline as preservative

expl. lim.

8.6 %

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

refractive index

n20/D 1.48

pH

~7.8(aqueous phase)

bp

182 °C (lit.)

mp

40-42 °C (lit.)

density

1.071 g/mL at 25 °C (lit.)

anion traces

sulfate (SO42-): ≤50 mg/kg

SMILES string

Oc1ccccc1

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

suitability

suitable for molecular biology

storage temp.

2-8°C

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

InChI key

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

Gene Information

human ... GABRA1(2554)

General description

saturated with 10 mM TRIS buffer pH 8.0 and 1 mM EDTA
Phenol, also known as hydroxybenzene, is an aromatic compound produced both naturally and synthetically. It acts as a disinfectant and is highly toxic. Exposure to phenol through inhalation, cutaneous adsorption, or oral ingestion causes denaturation and precipitation of cellular protein, which in turn leads to poisoning.

Application

Phenol (TE-saturated) has been used in the phenol-chloroform extraction procedure for plasmid DNA isolation. It has also been used to extract the aqueous phase during DNA linearization and mRNA synthesis.

Other Notes

RNA fingerprinting - preparation of the E. coli tRNA carrier

Disclaimer

according to temperature, a small quantity of an overlaid aqueous phase may form


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Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

target_organs

Nervous system,Kidney,Liver,Skin

Clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 2

flash_point_f

174.2 °F - closed cup

flash_point_c

79 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter



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Biodegradation of phenol: mechanisms and applications
van Schie PM and Young LY
Bioremediation Journal, 1222-1224 (2000)
Phenol and its toxicity: A case report
Chand M, et al.
Iranian Journal of Toxicology, 247-274 (2015)
Induction of Natural Competence in Genetically-modified Lactococcus lactis
Mulder J, et al.
Bio-protocol, 1-18 (2018)