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Merck

D8900

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

DMEM Hams F12, DME/F-12, 1:1 mixture

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UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:
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Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. It has been used:

  • as a component of the culture medium for endometrial explants in vitro culture
  • to immerse chitosan-gelatin-glycerol phosphate hydrogels for cytotoxicity evaluation
  • to culture nucleus pulposus (NP) cells to culture human articular chondrocytes for evaluating the toxicity of baicalein

Preparation Note

Formulated to contain 15.6 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate.


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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1



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