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G0282

Granulocyte-Macrophage Colony-Stimulating Factor from mouse

Name: Granulocyte-Macrophage Colony-Stimulating Factor from mouse
Brand: SIGMA

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sinónimos:

GM-CSF

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UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00241918

Biological source:

mouse

Recombinant:

expressed in E. coli

Assay:

≥97% (SDS-PAGE)

Form:

lyophilized powder

Mol wt:

~15 kDa (125 amino acids including N-terminal methionine)

Impurities:

≤1 EU/mg

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Propiedades

Nombre del producto

Granulocyte-Macrophage Colony-Stimulating Factor from mouse, GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biological source

mouse

Quality Segment

200

recombinant

expressed in E. coli

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

≤0.2 ng/mL EC₅₀ (corresponds to ≥5 × 10⁶ units/mg)

quality

endotoxin tested

mol wt

~15 kDa (125 amino acids including N-terminal methionine)

packaging

pkg of 5 and 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/mg

color

white

solubility

water: soluble, clear, colorless

UniProt accession no.

P01587

storage temp.

−20°C

Gene Information

mouse ... CSF2(12981), Csf2(12981)

Descripción

Biochem/physiol Actions

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

Physical form

Lyophilized from 10 mM acetic acid plus 250 μg BSA.

Analysis Note

The EC₅₀ activity of mouse GM-CSF is tested in culture using murine FDP-1 cells.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c