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Merck

H7033

PerfectHyb Plus Hybridization Buffer

for Northern and Southern blotting, solution

Sinónimos:

hyb buffer, hyb solution, hybridization solution, northern hybridization buffer, southern hybridization buffer

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NACRES:
NA.25
UNSPSC Code:
12161700
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General description

Sigma′s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours.

Application

Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots.

Features and Benefits

  • Fast and easy-to-use
  • Up to 20x more sensitive than conventional buffers
  • Doesn′t require blocking steps
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays
  • Compatible with radioactive and non-radioactive probes
  • Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes

Other Notes

A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion.

Legal Information

PerfectHyb is a trademark of Sigma-Aldrich Co. LLC

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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