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Merck

L3916

L-Lactic Dehydrogenase from bovine heart

1000 units/mL

Sinónimos:

Lactate, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD

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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-617-8
MDL number:
Número CE:
Specific activity:
1000 units/mL
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form

solution

Quality Level

specific activity

1000 units/mL

storage temp.

2-8°C

General description

LDH (lactic dehydrogenase) is a glycolytic enzyme. It is found in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It comprises of five isoenzyme forms. LDH possess a tetrameric structure.

Application

For use in enzymatic determination of lactate or pyruvate.
L-Lactic Dehydrogenase from bovine heart has been used:
  • as a reference standard to measure the LDH (lactic dehydrogenase)
  • to quench protein-bound NADH in cells
  • to measure the lactate released by the cells in suspension

Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
LDH (lactic dehydrogenase) helps in the reversible conversion of lactate to pyruvate.

Physical form

Suspension in 2.2 M ammonium sulfate

Other Notes

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)



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