L4402
Lisostafina from Staphylococcus staphylolyticus
BioUltra, ≥97% (SDS-PAGE), Protein 40-60 % by biuret, ≥2,000 units/mg protein
Sinónimos:
Endopeptidasa glicil-glicina
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥2,000 units/mg protein
Assay:
≥97% (SDS-PAGE)
Biological source:
bacterial (Staphylococcus sp.)
Concentration:
40—60% protein
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Permítanos ayudarlebiological source
bacterial (Staphylococcus sp.)
product line
BioUltra
assay
≥97% (SDS-PAGE)
form
lyophilized powder
specific activity
≥2,000 units/mg protein
mol wt
25 kDa
composition
Protein, 40-60% biuret
concentration
40—60% protein
technique(s)
cell based assay: suitable
suitability
suitable for cell lysis
antibiotic activity spectrum
Gram-positive bacteria
application(s)
diagnostic assay manufacturing
mode of action
cell wall synthesis | interferes
storage temp.
−20°C
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General description
Chemical structure: peptide
Package size based on protein content
Biochem/physiol Actions
La lisostafina es una endopeptidasa de cinc con un peso molecular de aproximadamente 25 kDa. Debido a que la lisostafina rompe los enlaces transversales de la poliglicina de la capa de peptidoglucanos de la pared celular de las especies de Staphylococcus , se ha encontrado útil para la lisis celular y también como potencial tratamiento antimicrobiano.
pH de actividad óptimo: ~7,5
pH de actividad óptimo: ~7,5
Physical form
Contains potassium phosphate buffer salts and sodium chloride
Preparation Note
Affinity purified
Other Notes
One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 °C in a 6.0 ml reaction mixture.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Johnny Gutierrez et al.
Open forum infectious diseases, 6(4), ofz090-ofz090 (2019-04-27)
Staphylococcus aureus (SA) bacteremia often requires a long treatment duration with antibiotics to prevent relapse due to the ability of SA to establish reservoirs of infection in sites such as heart and bone. These metastatic sites of infection cannot be
Igor Belyansky et al.
The Journal of surgical research, 171(2), 409-415 (2011-06-24)
Lysostaphin (LS), a naturally occurring Staphylococcal endopeptidase, has the ability to penetrate biofilm, and has been identified as a potential antimicrobial to prevent mesh infection. The goals of this study were to determine if LS adhered to porcine mesh (PM)
Fuying Cui et al.
Drug delivery, 17(8), 617-623 (2010-08-24)
Nasal colonization of Staphylococcus aureus (S.aureus) is known as a significant risk factor for nosocomial infections, and clearance of its nasal colonization greatly reduces the risk. In the present study the preparation and characterizations of the chitosan-o/w cream incorporated with
Martin Bjerregård Pedersen et al.
Journal of immunology (Baltimore, Md. : 1950), 184(4), 1931-1945 (2010-01-08)
The binding of Abs to microbial surfaces followed by complement activation constitutes an important line of defense against infections. In this study, we have investigated the relationship between complement activation and the binding of human IgM Abs to surfaces with
A P Desbois et al.
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 30(8), 1015-1021 (2011-02-12)
Drug-resistant staphylococci constitute a serious problem that urgently requires the discovery of new therapeutic agents. There has been a resurgence in interest in using lysostaphin (a specific anti-staphylococcal enzyme) as a treatment for infections caused by these important pathogens. However
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Instructions
QC Methods
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