P2949
PIPES sodium salt
≥99% (titration)
Sinónimos:
Na-PIPES, Piperazine-1,4-diethanesulfonate sodium salt, 1,4-Piperazinediethanesulfonic acid sodium salt, Piperazine-N,N′-bis(2-ethanesulfonic acid) sodium salt
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Fórmula lineal:
C8H17N2O6S2Na
Número CAS:
Peso molecular:
324.35
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
EC Number:
233-005-3
MDL number:
Servicio técnico
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Permítanos ayudarleQuality Level
assay
≥99% (titration)
form
crystalline powder
pH
4.5-4.7
useful pH range
6.1-7.5
pKa (25 °C)
6.8
solubility
0.1 M NaOH: 250 mg/mL, clear, colorless
application(s)
diagnostic assay manufacturing
storage temp.
room temp
SMILES string
[Na+].OS(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O
InChI
1S/C8H18N2O6S2.Na/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;/h1-8H2,(H,11,12,13)(H,14,15,16);/q;+1/p-1
InChI key
OGGAIRCLBMGXCZ-UHFFFAOYSA-M
General description
1, 4-piperazinediethanesulfonic acid (PIPES) is a zwitterionic buffer that belongs to the group of the ethanesulfonic acid buffer series, first introduced by Good et al., It is a non-toxic buffer used in cell culture and tissue fixation for electron microscopy and other biological applications. PIPES don’t interact with metal ions. Since its pKa at 37°C is near physiological pH, it has been used as a buffering substance in biological, biochemical, and environmental studies. PIPES is developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Buffers can be prepared by adding a solution of the base to PIPES-free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH.
Application
PIPES sodium salt buffer has been used:
- to prepare BRB80 buffer for imaging dynamic microtubules and single kinesin molecules through fluorescence microscopy
- as a component of PEM fixation solution
- to reconstitute red blood cells (RBCs) to study the effect of pH on 5-coordinate alpha-nitrosyl-hemoglobin (HbNO) stability
Features and Benefits
- Non-toxic
- Easy to use
- Thermally stable
- Easy to handle and store
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenido relacionado
Catalytic application of 1, 4-piperazinediethanesulfonic acid (PIPES) for the one-pot multicomponent synthesis of pyrano [4, 3-b] pyrans
Organic preparations and procedures international, 52(4), 368-373 (2020)
Conventional specimen preparation techniques for transmission electron microscopy of cultured cells
Bozzola JJ
Methods in Molecular Biology, 1-18 (2007)
Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
Tuna Y, et al.
Journal of Visualized Experiments (2022)