P4105
Pyruvate Oxidase from Aerococcus sp.
lyophilized powder, ≥35 units/mg protein (biuret)
Sinónimos:
Pyruvate: oxygen oxidoreductase (phosphorylating)
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-644-5
MDL number:
Specific activity:
≥35 units/mg protein (biuret)
Biological source:
bacterial (Aerococcus spp.)
Servicio técnico
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Permítanos ayudarlebiological source
bacterial (Aerococcus spp.)
Quality Level
form
lyophilized powder
specific activity
≥35 units/mg protein (biuret)
storage temp.
−20°C
Application
Pyruvate Oxidase from Aerococcus sp. has been used in the amplex Red-based fluorescence assay for pyruvate. It is suitable for use in the preparation of biosensor for determination of thiamine (vitamin B1), and as a component in the constant part of the multi-enzyme biocatalytic cascade for the determination of biomarkers for traumatic brain injury (TBI) and soft tissue injury (STI).
Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and CO2. It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarumand Streptococcus pneumoniae.
Biochem/physiol Actions
Pyruvate Oxidase oxidises pyruvate to form acetate, hydrogen peroxide and carbon dioxide. Pyruvate oxidase requires flavin adenine dinucleotide (FAD), thiamine pyrophosphate (TPP) and magnesium as cofactors for its catalytic activity. Thiamine activates pyruvate oxidase activity.
Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.
Physical form
Lyophilized powder containing buffer salt and sugar
Other Notes
One unit will produce 1.0 μmole of H2O2 per min during the conversion of pyruvate and phosphate to acetylphosphate and CO2 at pH 6.7 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Activation of pyruvate oxidase by monomeric and micellar amphiphiles.
R Blake et al.
The Journal of biological chemistry, 253(6), 1963-1971 (1978-03-25)
B Sedewitz et al.
Journal of bacteriology, 160(1), 273-278 (1984-10-01)
Pyruvate oxidase (EC 1.2.3.3) was isolated and characterized from Lactobacillus plantarum. The enzyme catalyzes the oxidative decarboxylation of pyruvate in the presence of phosphate and oxygen, yielding acetyl phosphate, carbon dioxide, and hydrogen peroxide. This pyruvate oxidase is a flavoprotein
Multi-enzyme logic network architectures for assessing injuries: digital processing of biomarkers
Halamek J, et al.
Molecular Biosystems, 6(12), 2554-2560 (2010)