SAB4301138
Anti-GFP antibody produced in rabbit
affinity isolated antibody
Sinónimos:
GFP-like chromoprotein
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NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Technique(s):
western blot: 1:1000-1:10000 (Cell Lysate)
Application:
WB
Citations:
61
Servicio técnico
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Permítanos ayudarlebiological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
27 kDa
concentration
3.2 mg/mL
technique(s)
western blot: 1:1000-1:10000 (Cell Lysate)
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Green fluorescent protein (GFP) is a 27kDa protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca2+- activated photoprotein aequorin to GFP.
Immunogen
Full length fusion protein
Application
Anti-GFP antibody produced in rabbit has been used in:
- double immunofluorescence staining
- immunoblot analysis.
- immunoprecipitation.
Biochem/physiol Actions
Green fluorescent protein (GFP) is a reporter molecule which is used for checking gene expression and protein localization in vivo, in situ and in real time. GFP emits green light when it is excited with UV/blue light. The GFP fluorescence remains stable and can be detected non-invasively in living cells. GFP is considered as a unique tool to monitor dynamic processes in several living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence.
The antibody detects transfected proteins containing GFP tag.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Green fluorescent protein as a reporter for macromolecular localization in bacterial cells.
Margolin W
Methods, 20(1), 62-72 (2000)
David A Rhodes et al.
Frontiers in immunology, 9, 662-662 (2018-04-20)
Activation of human Vγ9/Vδ2 T cells by "phosphoantigens" (pAg), the microbial metabolite (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP) and the endogenous isoprenoid intermediate isopentenyl pyrophosphate, requires expression of butyrophilin BTN3A molecules by presenting cells. However, the precise mechanism of activation of Vγ9/Vδ2 T
UGT79B31 is responsible for the final modification step of pollen-specific flavonoid biosynthesis in Petunia hybrida.
Knoch E, et al.
Planta, 247(4), 779-790 (2018)