SRP0229
WWP1 Active human
recombinant, expressed in baculovirus infected insect cells, ≥15% (SDS-PAGE)
Sinónimos:
AIP5, Atrophin-1 interacting protein 5, NEDD4-like E3 ubiquitin-protein ligase, TGIF-interacting ubiquitin ligase 1 (Tiul1), WW domain containing E3 ubiquitin protein ligase 1
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NACRES:
NA.32
UNSPSC Code:
12352200
Assay:
≥15% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected insect cells
Concentration:
>0.02 mg/mL
Servicio técnico
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Permítanos ayudarlebiological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥15% (SDS-PAGE)
form
aqueous solution
mol wt
106 kDa
packaging
pkg of 20 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... WWP1(11059)
General description
Human WWP1 (GenBank Accession No. NM_007013), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C.
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 2
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Yu-Ru Lee et al.
Science (New York, N.Y.), 364(6441) (2019-05-18)
Activation of tumor suppressors for the treatment of human cancer has been a long sought, yet elusive, strategy. PTEN is a critical tumor suppressive phosphatase that is active in its dimer configuration at the plasma membrane. Polyubiquitination by the ubiquitin