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T7024

N,N,N′,N′-Tetrametiletilendiamina

BioReagent, Molecular Biology, ≥99% (GC)

Sinónimos:

1,2- Bis(dimetilamino)-etano, TEMED, TMEDA

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Fórmula lineal:
(CH3)2NCH2CH2N(CH3)2
Número CAS:
110-18-9
Peso molecular:
116.20
UNSPSC Code:
41105319
NACRES:
NA.31
PubChem Substance ID:
24900242
EC Number:
203-744-6
Beilstein/REAXYS Number:
1732991
MDL number:
MFCD00008335
Assay:
≥99% (GC)
Grade:
Molecular Biology
Technique(s):
electrophoresis: suitable
Bp:
120-122 °C (lit.)
Vapor pressure:
ca.19.19 hPa
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grade

Molecular Biology

vapor pressure

ca.19.19 hPa

product line

BioReagent

assay

≥99% (GC)

expl. lim.

9.08 %

technique(s)

electrophoresis: suitable

refractive index

n20/D 1.4179 (lit.)

bp

120-122 °C (lit.)

mp

−55 °C (lit.)

density

0.775 g/mL at 20 °C (lit.)

foreign activity

DNase, RNase, protease, none detected

SMILES string

CN(C)CCN(C)C

InChI

1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3

InChI key

KWYHDKDOAIKMQN-UHFFFAOYSA-N

Application

This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.,,
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied. TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED. TEMED has been used in the HPLC of small organic molecules.

Preparation Note

Suggested use is 35 μl for 50 ml acrylamide gel solution.

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Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Clase de almacenamiento

3 - Flammable liquids

wgk

WGK 1

flash_point_f

61.7 °F - closed cup

flash_point_c

16.5 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
BCCN8929
BCCM7546
BCCL1561
BCCK6320
BCCJ9691

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Andrews, A. T.
Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications, 21-24 (1986)
A V Vasiliskov et al.
BioTechniques, 27(3), 592-594 (1999-09-18)
The manufacturing of microchips containing oligonucleotides and proteins immobilized within gel pads, ranging in size from 10 x 10 to 100 x 100 microns, is described. The microchips are produced by photo- or persulfate-induced copolymerization of unsaturated derivatives of biomolecules
Sambrook, J. and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 5-5 (2001)
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